Figure 4

Effects of EPA and OEPA on the expression of sterol-regulatory element binding protein-1 (SREBP-1). A: Effect on SREBP-1c mRNA expression. Each fatty acid (60 μM) was added to HepG2 cells with T0901317 (10 nM) and vitamin E (10 μM) as noted in serum-free medium containing 0.1% BSA. The final ethanol concentration was 0.3%. After 24 h incubation, SREBP-1c mRNA levels were quantified by real-time quantitative PCR. Each value of SREBP-1c mRNA was adjusted by that of 18 s rRNA (internal control). Expression levels are presented as -fold induction relative to the vehicle control (vitamin E and ethanol). Data are reported as means ± SD (n = 3/group). Values with different letters are significantly different (p < 0.05). B,C: Effects on precursor and mature SREBP-1 protein expression. Each fatty acid was added to HepG2 cells with T0901317 (10 nM) and vitamin E (10 μM) as noted in serum-free medium containing 0.1% BSA. The final ethanol concentration was 0.3%. Representative Western blots are shown. The data represent the mean -fold change of the precursor and the mature forms of SREBP-1 from the vehicle control (ethanol). Data are reported as means ± SD (n = 3-4/group). Values with different letters are significantly different (p < 0.05).