Effects of EPA, 5-HEPE, 11-HEPE and 18-HEPE on the expression of sterol-regulatory element binding protein-1c (SREBP-1c; A), acetyl-CoA carboxylase-1 (ACC; B), fatty acid synthase (FAS; C), stearoyl-coenzyme A desaturase-1 (SCD-1; D), glycerol-3-phosphate acyltransferase (GPA; E) and peroxisome proliferator-activated receptor γ coactivator 1β (PGC-1β; F) mRNAs. Each fatty acid (60 μM) was added to HepG2 cells with T0901317 (10 nM) and vitamin E (10 μM) as noted in serum-free medium containing 0.1% BSA. The final ethanol concentration was 0.3%. After 24 h incubation, SREBP-1c, ACC, FAS, SCD-1, GPA and PGC-1β mRNA levels were quantified by real-time quantitative PCR. The value of each mRNA was adjusted by that of 18 s rRNA (internal control). Expression levels are presented as -fold induction relative to the vehicle control (vitamin E and ethanol). Data are reported as means ± SD (n = 3/group). * A significant difference from the T0901317 vehicle control group (p < 0.05).