Figure 2

The effects of repeated exposure to growth media on de novo lipogenesis and lipid storage. Differentiated 3 T3-L1 adipocytes in the CON (open bar) and RF (closed bar) groups (day 9) were incubated from ZT 15 to ZT 19 for 4 consecutive days with either growth medium (L-DM), growth medium with 2 mM oleate (L-DMOL), or DMEM with high glucose/10% FBS supplemented with 5 μg/ml insulin and 2 mM oleate (H-DMOL). TAG content was then measured using a TG E-test kit (Wako). * p < 0.05 vs. CON group.