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Table 6 In vivo and in vitro Cu 2+ -induced generation of malondialdehyde (MDA) in plasma LDL, sd-LDL and lb-LDL from rats after 30 days of methanolic extract (ME) and volatile oil (VO) treatment

From: Elucidation of mechanisms of actions of thymoquinone-enriched methanolic and volatile oil extracts from Nigella sativa against cardiovascular risk parameters in experimental hyperlipidemia

Group Malondialdehyde
  LDL Sd-LDL Lb-LDL
  Basal Maximal Basal Maximal Basal Maximal
NLP-C 3.14 ± 0.05 26.69 ± 0.11 (+750.00%) 4.71 ± 0.02 33.02 ± 0.09 (+601.06%) 3.83 ± 0.08 24.73 ± 0.05 (+549.69%)
HLP-C 6.47 ± 0.04 (+106.05%)a 48.40 ± 0.09 (+81.31%)a 8.05 ± 0.06 (+70.91%)a 55.60 ± 0.05 (+66.38%)a 6.18 ± 0.07 (+61.35%)a 32.68 ± 0.06 (+32.14%)a
HLP- ME 4.05 ± 0.08 (-37.40%)b 32.02 ± 0.06 (-33.84%)b 5.78 ± 0.05 (-28.19%)b 39.37 ± 0.06 (-29.19%)b 4.79 ± 0.09 (-22.49%)b 26.73 ± 0.07 (-18.20%)b
HLP-VO 4.80 ± 0.06 (-25.81%)b 36.98 ± 0.03 (-23.59%)b 6.92 ± 0.05 (-14.03%)b 47.08 ± 0.07 (-15.32%)b 5.30 ± 0.04 (-14.23%)b 28.30 ± 0.05 (-13.40%)b
  1. The values are expressed as nmole/mg protein. The basal MDA values in the table show the in vivo status of oxidized LDL, and its subfractions sd-LDL or lb-LDL. In the case of maximal MDA values of the lipoproteins (LDL and lb-LDL oxidation) were achieved after 2 h of incubation with CuSO4, whereas for sd-LDL maximal values were obtained after 30 min. Each value represents mean ± SD from LDL, sd-LDL and lb-LDL subfractions, isolated from pooled plasma samples in each group. The values were statistically significant from NLP-C at ap < 0.05 and HLP-C at bp < 0.05, and the values between the treated groups were also significant (p < 0.05).