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Table 3 Selected fatty acids in lipids of the mammary gland at 42 DIM and 105 DIM of supplementation

From: A commonly used rumen-protected conjugated linoleic acid supplement marginally affects fatty acid distribution of body tissues and gene expression of mammary gland in heifers during early lactation

Fatty acid [% FAME] Period 1 (42 DIM) Period 2 (105 DIM)
CON CLA CON CLA
  Mean ± SD Mean ± SD Mean ± SD Mean ± SD
14:0 9.88 ± 0.70 9.40 ± 2.10 9.10 ± 0.87 9.13 ± 1.67
c 9-14:1 0.65 ± 0.19 0.51 ± 0.19 0.83 ± 0.02 0.68 ± 0.27
16:0 32.62 ± 3.16 30.16 ± 4.28 31.41 ± 3.64 29.05 ± 3.13
c 9-16:1 1.52 ± 0.16 1.49 ± 0.12 1.84 ± 0.10 1.89 ± 0.25
18:0 10.38 ± 1.43 11.84 ± 2.56 9.80 ± 2.00 11.17 ± 2.05
t 6/t 7/t 8-18:1 0.08 ± 0.05 0.14 ± 0.02* 0.13 ± 0.02 0.17 ± 0.07
t 9-18:1 0.20 ± 0.01 0.25 ± 0.02* 0.23 ± 0.01 0.28 ± 0.06
t 10-18:1 0.22 ± 0.06 0.37 ± 0.09* 0.29 ± 0.05 0.68 ± 0.68
t 11-18:1 0.65 ± 0.13 0.77 ± 0.04 0.68 ± 0.06 0.89 ± 0.37
t 12-18:1 0.21 ± 0.03 0.29 ± 0.02* 0.27 ± 0.02 0.31 ± 0.05
t 13/t 14-18:1 ND ND ND ND
c 9-18:1 20.45 ± 2.88 22.46 ± 3.11 22.34 ± 2.56 22.82 ± 5.00
c 11-18:1 0.88 ± 0.13 1.06 ± 0.11* 0.84 ± 0.17 1.12 ± 0.24
c 12-18:1 0.22 ± 0.03 0.31 ± 0.01* 0.28 ± 0.02 0.33 ± 0.04*
c 13-18:1 0.08 ± 0.02 0.10 ± 0.01 0.10 ± 0.02 0.11 ± 0.02
18:2 n-6 2.52 ± 0.40 3.43 ± 0.57* 3.08 ± 0.27 4.02 ± 1.23
18:3 n-3 0.42 ± 0.09 0.53 ± 0.09 0.41 ± 0.03 0.49 ± 0.10
20:4 n-6 0.37 ± 0.08 0.46 ± 0.11 0.48 ± 0.06 0.65 ± 0.14
20:5 n-3 0.13 ± 0.03 0.17 ± 0.05 0.12 ± 0.01 0.16 ± 0.03*
22:5 n-3 0.25 ± 0.05 0.32 ± 0.10 0.27 ± 0.03 0.33 ± 0.07
22:6 n-3 0.03 ± 0.01 0.03 ± 0.01 0.02 ± 0.01 0.03 ± 0.01
CLA
  c 9,t 11-CLA 0.37 ± 0.03 0.49 ± 0.11 0.51 ± 0.06 0.73 ± 0.34
  t 10,c 12-CLA ND 0.05 ± 0.01 ND 0.05 ± 0.01
  Other CLA 0.06 ± 0.02 0.09 ± 0.01* 0.07 ± 0.02 0.10 ± 0.04
Summations
  ∑ SFA 69.04 ± 3.20 64.84 ± 3.74 65.13 ± 2.65 62.13 ± 5.51
  ∑ MUFA 26.33 ± 2.89 29.02 ± 2.90 29.12 ± 2.76 30.59 ± 4.73
  ∑ PUFA n-3 0.91 ± 0.14 1.17 ± 0.25 0.93 ± 0.05 1.12 ± 0.18
  ∑ PUFA n-6 3.29 ± 0.55 4.34 ± 0.71* 4.23 ± 0.33 5.27 ± 1.41
  ∑ PUFA incl. CLA 4.64 ± 0.69 6.14 ± 0.86* 5.74 ± 0.39 7.28 ± 1.94
  ∑ trans-18:1 1.81 ± 0.34 2.37 ± 0.20* 2.11 ± 0.15 2.87 ± 1.25
Ratio
  c 9-14:1/14:0 0.07 ± 0.02 0.05 ± 0.01 0.09 ± 0.01 0.07 ± 0.02
  1. Cows in the control (CON) group (n = 5) of each period received a control fat supplement, in which the CLA were substituted with stearic acid. Cows in the conjugated linoleic acid (CLA) group (n = 5) of each period consumed 6 g/d each of trans-10,cis-12 CLA and cis-9,trans-11 CLA.
  2. *Means ± SD for each period are defined significant (P < 0.05).
  3. ND not detected.