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Table 3 Selected fatty acids in lipids of the mammary gland at 42 DIM and 105 DIM of supplementation

From: A commonly used rumen-protected conjugated linoleic acid supplement marginally affects fatty acid distribution of body tissues and gene expression of mammary gland in heifers during early lactation

Fatty acid [% FAME]

Period 1 (42 DIM)

Period 2 (105 DIM)

CON

CLA

CON

CLA

 

Mean ± SD

Mean ± SD

Mean ± SD

Mean ± SD

14:0

9.88 ± 0.70

9.40 ± 2.10

9.10 ± 0.87

9.13 ± 1.67

c 9-14:1

0.65 ± 0.19

0.51 ± 0.19

0.83 ± 0.02

0.68 ± 0.27

16:0

32.62 ± 3.16

30.16 ± 4.28

31.41 ± 3.64

29.05 ± 3.13

c 9-16:1

1.52 ± 0.16

1.49 ± 0.12

1.84 ± 0.10

1.89 ± 0.25

18:0

10.38 ± 1.43

11.84 ± 2.56

9.80 ± 2.00

11.17 ± 2.05

t 6/t 7/t 8-18:1

0.08 ± 0.05

0.14 ± 0.02*

0.13 ± 0.02

0.17 ± 0.07

t 9-18:1

0.20 ± 0.01

0.25 ± 0.02*

0.23 ± 0.01

0.28 ± 0.06

t 10-18:1

0.22 ± 0.06

0.37 ± 0.09*

0.29 ± 0.05

0.68 ± 0.68

t 11-18:1

0.65 ± 0.13

0.77 ± 0.04

0.68 ± 0.06

0.89 ± 0.37

t 12-18:1

0.21 ± 0.03

0.29 ± 0.02*

0.27 ± 0.02

0.31 ± 0.05

t 13/t 14-18:1

ND

ND

ND

ND

c 9-18:1

20.45 ± 2.88

22.46 ± 3.11

22.34 ± 2.56

22.82 ± 5.00

c 11-18:1

0.88 ± 0.13

1.06 ± 0.11*

0.84 ± 0.17

1.12 ± 0.24

c 12-18:1

0.22 ± 0.03

0.31 ± 0.01*

0.28 ± 0.02

0.33 ± 0.04*

c 13-18:1

0.08 ± 0.02

0.10 ± 0.01

0.10 ± 0.02

0.11 ± 0.02

18:2 n-6

2.52 ± 0.40

3.43 ± 0.57*

3.08 ± 0.27

4.02 ± 1.23

18:3 n-3

0.42 ± 0.09

0.53 ± 0.09

0.41 ± 0.03

0.49 ± 0.10

20:4 n-6

0.37 ± 0.08

0.46 ± 0.11

0.48 ± 0.06

0.65 ± 0.14

20:5 n-3

0.13 ± 0.03

0.17 ± 0.05

0.12 ± 0.01

0.16 ± 0.03*

22:5 n-3

0.25 ± 0.05

0.32 ± 0.10

0.27 ± 0.03

0.33 ± 0.07

22:6 n-3

0.03 ± 0.01

0.03 ± 0.01

0.02 ± 0.01

0.03 ± 0.01

CLA

  c 9,t 11-CLA

0.37 ± 0.03

0.49 ± 0.11

0.51 ± 0.06

0.73 ± 0.34

  t 10,c 12-CLA

ND

0.05 ± 0.01

ND

0.05 ± 0.01

  Other CLA

0.06 ± 0.02

0.09 ± 0.01*

0.07 ± 0.02

0.10 ± 0.04

Summations

  ∑ SFA

69.04 ± 3.20

64.84 ± 3.74

65.13 ± 2.65

62.13 ± 5.51

  ∑ MUFA

26.33 ± 2.89

29.02 ± 2.90

29.12 ± 2.76

30.59 ± 4.73

  ∑ PUFA n-3

0.91 ± 0.14

1.17 ± 0.25

0.93 ± 0.05

1.12 ± 0.18

  ∑ PUFA n-6

3.29 ± 0.55

4.34 ± 0.71*

4.23 ± 0.33

5.27 ± 1.41

  ∑ PUFA incl. CLA

4.64 ± 0.69

6.14 ± 0.86*

5.74 ± 0.39

7.28 ± 1.94

  ∑ trans-18:1

1.81 ± 0.34

2.37 ± 0.20*

2.11 ± 0.15

2.87 ± 1.25

Ratio

  c 9-14:1/14:0

0.07 ± 0.02

0.05 ± 0.01

0.09 ± 0.01

0.07 ± 0.02

  1. Cows in the control (CON) group (n = 5) of each period received a control fat supplement, in which the CLA were substituted with stearic acid. Cows in the conjugated linoleic acid (CLA) group (n = 5) of each period consumed 6 g/d each of trans-10,cis-12 CLA and cis-9,trans-11 CLA.
  2. *Means ± SD for each period are defined significant (P < 0.05).
  3. ND not detected.