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Table 4 Selected fatty acids in liver lipids at 42 DIM and 105 DIM of supplementation

From: A commonly used rumen-protected conjugated linoleic acid supplement marginally affects fatty acid distribution of body tissues and gene expression of mammary gland in heifers during early lactation

Fatty acid [% FAME] Period 1 (42 DIM) Period 2 (105 DIM)
  CON CLA CON CLA
  Mean ± SD Mean ± SD Mean ± SD Mean ± SD
14:0 1.18 ± 0.69 1.46 ± 0.43 0.69 ± 0.24 0.67 ± 0.17
c 9-14:1 0.19 ± 0.13 0.18 ± 0.14 0.16 ± 0.11 0.07 ± 0.08
16:0 14.37 ± 4.59 13.95 ± 1.92 10.93 ± 1.54 11.42 ± 3.75
c 9-16:1 1.35 ± 0.77 1.36 ± 0.46 0.76 ± 0.31 0.65 ± 0.31
18:0 28.41 ± 4.93 28.44 ± 2.35 32.34 ± 1.16 31.47 ± 4.48
t 6/t 7/t 8-18:1 0.03 ± 0.02 0.05 ± 0.01 0.04 ± 0.01 0.05 ± 0.03
t 9-18:1 0.13 ± 0.02 0.16 ± 0.02 0.15 ± 0.03 0.16 ± 0.07
t 10-18:1 0.08 ± 0.02 0.15 ± 0.02* 0.08 ± 0.03 0.30 ± 0.42
t 11-18:1 0.56 ± 0.11 0.74 ± 0.13 0.49 ± 0.08 0.80 ± 0.52
t 12-18:1 0.22 ± 0.08 0.31 ± 0.04 0.24 ± 0.04 0.27 ± 0.03
t 13/t 14-18:1 0.60 ± 0.44 0.50 ± 0.14 0.79 ± 0.05 0.62 ± 0.38
c 9-18:1 15.31 ± 4.49 14.21 ± 2.28 11.96 ± 0.81 12.10 ± 5.46
c 11-18:1 1.79 ± 0.10 1.92 ± 0.12 1.56 ± 0.15 1.60 ± 0.30
c 12-18:1 0.18 ± 0.02 0.29 ± 0.05* 0.18 ± 0.04 0.27 ± 0.07*
c 13-18:1 0.15 ± 0.01 0.17 ± 0.05 0.19 ± 0.04 0.16 ± 0.06
18:2 n-6 9.04 ± 0.67 9.93 ± 0.25* 9.06 ± 1.17 11.13 ± 1.12*
18:3 n-3 0.83 ± 0.30 0.99 ± 0.27 0.58 ± 0.09 0.79 ± 0.23
20:4 n-6 5.43 ± 0.75 5.37 ± 1.05 6.10 ± 0.72 6.02 ± 0.58
20:5 n-3 1.50 ± 0.47 1.55 ± 0.21 1.24 ± 0.13 1.29 ± 0.43
22:5 n-3 3.70 ± 0.81 3.76 ± 0.58 4.12 ± 0.44 4.12 ± 0.97
22:6 n-3 0.62 ± 0.09 0.79 ± 0.11* 0.43 ± 0.07 0.53 ± 0.14
CLA
  c 9,t 11-CLA 0.24 ± 0.10 0.29 ± 0.07 0.19 ± 0.06 0.26 ± 0.13
  t 10,c 12-CLA ND ND ND ND
  Other CLA 0.14 ± 0.10 0.12 ± 0.05 0.16 ± 0.02 0.10 ± 0.06
Summations
  ∑ SFA 48.95 ±1.11 49.08 ± 0.57 49.08 ± 0.97 48.62 ± 1.75
  ∑ MUFA 22.03 ± 5.11 21.58 ± 3.03 17.99 ± 1.21 18.57 ± 5.26
  ∑ PUFA n-3 7.71 ± 1.41 8.34 ± 0.48 7.68 ± 0.66 7.84 ± 1.58
  ∑ PUFA n-6 20.92 ± 3.74 20.59 ± 2.30 24.90 ± 1.26 24.60 ± 2.77
  ∑ PUFA incl. CLA 29.02 ± 4.96 29.34 ± 2.50 32.93 ± 1.79 32.81 ± 3.89
  ∑ trans-18:1 2.10 ± 0.43 2.51 ± 0.17 2.42 ± 0.17 2.95 ± 1.33
Ratio
  c 9-14:1/14:0 0.18 ± 0.12 0.11 ± 0.06 0.21 ± 0.11 0.11 ± 0.10
  1. Cows in the control (CON) group (n = 5) of each period received a control fat supplement, in which the CLA were substituted with stearic acid. Cows in the conjugated linoleic acid (CLA) group (n = 5) of each period consumed 6 g/d each of trans-10,cis-12 CLA and cis-9,trans-11 CLA.
  2. *Means ± SD for each period are defined significant (P < 0.05).
  3. ND not detected.