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Table 5 Selected fatty acids in lipids of M . longissimus at 42 DIM and 105 DIM of supplementation

From: A commonly used rumen-protected conjugated linoleic acid supplement marginally affects fatty acid distribution of body tissues and gene expression of mammary gland in heifers during early lactation

Fatty acid [% FAME] Period 1 (42 DIM) Period 2 (105 DIM)
  CON CLA CON CLA
  Mean ± SD Mean ± SD Mean ± SD Mean ± SD
14:0 1.73 ± 0.37 1.79 ± 0.28 1.74 ± 0.31 1.67 ± 0.52
c 9-14:1 0.49 ± 0.20 0.41 ± 0.05 0.57 ± 0.20 0.36 ± 0.25
16:0 25.42 ± 2.39 25.38 ± 1.52 25.55 ± 1.38 25.09 ± 2.32
c 9-16:1 3.74 ± 1.01 3.75 ± 0.48 4.11 ± 0.80 3.61 ± 1.15
18:0 13.54 ± 1.31 13.38 ± 0.82 12.07 ± 1.26 13.59 ± 1.68
t 6/t 7/t 8-18:1 0.12 ± 0.02 0.12 ± 0.02 0.14 ± 0.01 0.13 ± 0.02
t 9-18:1 0.30 ± 0.02 0.31 ± 0.04 0.32 ± 0.02 0.32 ± 0.03
t 10-18:1 0.24 ± 0.02 0.26 ± 0.01 0.29 ± 0.04 0.36 ± 0.07
t 11-18:1 0.52 ± 0.05 0.54 ± 0.09 0.52 ± 0.05 0.53 ± 0.10
t 12-18:1 0.13 ± 0.02 0.13 ± 0.01 0.14 ± 0.01 0.15 ± 0.02
t 13/t 14-18:1 ND ND ND ND
c 9-18:1 43.01 ± 1.76 40.94 ± 3.22 43.41 ± 1.38 41.04 ± 1.76*
c 11-18:1 1.81 ± 0.22 1.90 ± 0.19 2.09 ± 0.34 1.78 ± 0.17
c 12-18:1 0.12 ± 0.05 0.12 ± 0.04 0.13 ± 0.03 0.14 ± 0.05
c 13-18:1 0.27 ± 0.05 0.20 ± 0.02 0.30 ± 0.08 0.18 ± 0.08*
18:2 n-6 2.29 ± 0.52 3.18 ± 1.36 2.53 ± 1.04 3.22 ± 1.14
18:3 n-3 0.36 ± 0.09 0.52 ± 0.17 0.34 ± 0.08 0.43 ± 0.12
20:4 n-6 0.66 ± 0.18 1.04 ± 0.69 0.69 ± 0.38 1.15 ± 0.74
20:5 n-3 0.14 ± 0.05 0.29 ± 0.23 0.14 ± 0.10 0.26 ± 0.18
22:5 n-3 0.25 ± 0.06 0.42 ± 0.23 0.24 ± 0.16 0.43 ± 0.24
22:6 n-3 ND 0.03 ± 0.03 ND 0.02 ± 0.02
CLA
  c 9,t 11-CLA 0.19 ± 0.03 0.19 ± 0.04 0.19 ± 0.03 0.21 ± 0.03
  t 10,c 12-CLA ND ND ND ND
  Other CLA 0.07 ± 0.01 0.07 ± 0.02 0.08 ± 0.01 0.08 ± 0.02
Summations
  ∑ SFA 44.32 ± 1.21 44.58 ± 1.57 42.86 ± 0.98 44.48 ± 0.75*
  ∑ MUFA 51.30 ± 1.09 49.11 ± 2.99 52.42 ± 2.18 49.08 ± 2.11*
  ∑ PUFA n-3 0.82 ± 0.18 1.35 ± 0.69 0.79 ± 0.36 1.22 ± 0.60
  ∑ PUFA n-6 3.30 ± 0.71 4.69 ± 2.27 3.65 ± 1.51 4.91 ± 2.15
  ∑ PUFA incl. CLA 4.38 ± 0.91 6.31 ± 2.95 4.71 ± 1.82 6.44 ± 2.76
  ∑ trans-18:1 1.46 ± 0.13 1.46 ± 0.12 1.49 ± 0.04 1.60 ± 0.22
Ratio
  c 9-14:1/14:0 0.27 ± 0.06 0.23 ± 0.03 0.32 ± 0.07 0.20 ± 0.07*
  1. Cows in the control (CON) group (n = 5) of each period received a control fat supplement, in which the CLA were substituted with stearic acid. Cows in the conjugated linoleic acid (CLA) group (n = 5) of each period consumed 6 g/d each of trans-10,cis-12 CLA and cis-9,trans-11 CLA.
  2. *Means ± SD for each period are defined significant (P < 0.05).
  3. ND not detected.