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Table 5 Selected fatty acids in lipids of M . longissimus at 42 DIM and 105 DIM of supplementation

From: A commonly used rumen-protected conjugated linoleic acid supplement marginally affects fatty acid distribution of body tissues and gene expression of mammary gland in heifers during early lactation

Fatty acid [% FAME]

Period 1 (42 DIM)

Period 2 (105 DIM)

 

CON

CLA

CON

CLA

 

Mean ± SD

Mean ± SD

Mean ± SD

Mean ± SD

14:0

1.73 ± 0.37

1.79 ± 0.28

1.74 ± 0.31

1.67 ± 0.52

c 9-14:1

0.49 ± 0.20

0.41 ± 0.05

0.57 ± 0.20

0.36 ± 0.25

16:0

25.42 ± 2.39

25.38 ± 1.52

25.55 ± 1.38

25.09 ± 2.32

c 9-16:1

3.74 ± 1.01

3.75 ± 0.48

4.11 ± 0.80

3.61 ± 1.15

18:0

13.54 ± 1.31

13.38 ± 0.82

12.07 ± 1.26

13.59 ± 1.68

t 6/t 7/t 8-18:1

0.12 ± 0.02

0.12 ± 0.02

0.14 ± 0.01

0.13 ± 0.02

t 9-18:1

0.30 ± 0.02

0.31 ± 0.04

0.32 ± 0.02

0.32 ± 0.03

t 10-18:1

0.24 ± 0.02

0.26 ± 0.01

0.29 ± 0.04

0.36 ± 0.07

t 11-18:1

0.52 ± 0.05

0.54 ± 0.09

0.52 ± 0.05

0.53 ± 0.10

t 12-18:1

0.13 ± 0.02

0.13 ± 0.01

0.14 ± 0.01

0.15 ± 0.02

t 13/t 14-18:1

ND

ND

ND

ND

c 9-18:1

43.01 ± 1.76

40.94 ± 3.22

43.41 ± 1.38

41.04 ± 1.76*

c 11-18:1

1.81 ± 0.22

1.90 ± 0.19

2.09 ± 0.34

1.78 ± 0.17

c 12-18:1

0.12 ± 0.05

0.12 ± 0.04

0.13 ± 0.03

0.14 ± 0.05

c 13-18:1

0.27 ± 0.05

0.20 ± 0.02

0.30 ± 0.08

0.18 ± 0.08*

18:2 n-6

2.29 ± 0.52

3.18 ± 1.36

2.53 ± 1.04

3.22 ± 1.14

18:3 n-3

0.36 ± 0.09

0.52 ± 0.17

0.34 ± 0.08

0.43 ± 0.12

20:4 n-6

0.66 ± 0.18

1.04 ± 0.69

0.69 ± 0.38

1.15 ± 0.74

20:5 n-3

0.14 ± 0.05

0.29 ± 0.23

0.14 ± 0.10

0.26 ± 0.18

22:5 n-3

0.25 ± 0.06

0.42 ± 0.23

0.24 ± 0.16

0.43 ± 0.24

22:6 n-3

ND

0.03 ± 0.03

ND

0.02 ± 0.02

CLA

  c 9,t 11-CLA

0.19 ± 0.03

0.19 ± 0.04

0.19 ± 0.03

0.21 ± 0.03

  t 10,c 12-CLA

ND

ND

ND

ND

  Other CLA

0.07 ± 0.01

0.07 ± 0.02

0.08 ± 0.01

0.08 ± 0.02

Summations

  ∑ SFA

44.32 ± 1.21

44.58 ± 1.57

42.86 ± 0.98

44.48 ± 0.75*

  ∑ MUFA

51.30 ± 1.09

49.11 ± 2.99

52.42 ± 2.18

49.08 ± 2.11*

  ∑ PUFA n-3

0.82 ± 0.18

1.35 ± 0.69

0.79 ± 0.36

1.22 ± 0.60

  ∑ PUFA n-6

3.30 ± 0.71

4.69 ± 2.27

3.65 ± 1.51

4.91 ± 2.15

  ∑ PUFA incl. CLA

4.38 ± 0.91

6.31 ± 2.95

4.71 ± 1.82

6.44 ± 2.76

  ∑ trans-18:1

1.46 ± 0.13

1.46 ± 0.12

1.49 ± 0.04

1.60 ± 0.22

Ratio

  c 9-14:1/14:0

0.27 ± 0.06

0.23 ± 0.03

0.32 ± 0.07

0.20 ± 0.07*

  1. Cows in the control (CON) group (n = 5) of each period received a control fat supplement, in which the CLA were substituted with stearic acid. Cows in the conjugated linoleic acid (CLA) group (n = 5) of each period consumed 6 g/d each of trans-10,cis-12 CLA and cis-9,trans-11 CLA.
  2. *Means ± SD for each period are defined significant (P < 0.05).
  3. ND not detected.