Figure 4

Autophagy altered the expression of ADRP in LPS-stimulated THP-1 macorphages. (A, B) Macrophages were incubated with LPS for a different time (0, 8, 16, and 24 h) or with different amounts of LPS (10-1000 ng/ml) for 24 h. Real-time PCR analysis ADRP gene expression. (C) THP-1 cells were treated with 1 μg/ml LPS in the absence or presence of Rap (10 μM), or 3MA (10 μM) as noted for 24 h. The level of ADRP was then quantified by real-time PCR. (D) The levels of ADRP and β-actin proteins were detected by using immunoblotting with specific antibodies. Results were presented as mean ± S.E. of 3 independent experiments. *significant difference from LPS treatment P < 0.05; **, P < 0.01.