Skip to main content
Figure 4 | Lipids in Health and Disease

Figure 4

From: ABCC6- a new player in cellular cholesterol and lipoprotein metabolism?

Figure 4

Gene expression analysis of cholesterol biosynthesis II: LSS, TM7SF2 and DHCR7. Quantification of (a, b) Lanosterol synthase (LSS), (c, d) transmembrane 7 superfamily member 2 (TM7SF2) and (e, f) 7-dehydrocholesterol reductase (DHCR7) mRNA expression in human dermal fibroblasts from healthy controls (n = 4; white), PXE patients (n = 4; black), scramble siRNA-negative control (siNK, n = 4; white-striped) and ABCC6-specific siRNA-treated cells (siABCC6, n = 4; black-shaded) grown for 24 h under different cell culture conditions (+10% FCS, +10% LPDS, −FCS). Expression levels are normalized to reference gene expressions (ACTB, GAPDH, β2M). Data are presented in arbitrary units as means with corresponding standard error. Control: PXE ratio/siABCC6: siNK ratio: ***p < 0.0005; **p < 0.005; *p < 0.03; Control 10% FCS: 10% LPDS, −FCS ratio/siNK 10% FCS: 10% LPDS, −FCS ratio: +++p < 0.0001; PXE 10% FCS: 10% LPDS, −FCS ratio/siABCC6 10% FCS: 10% LPDS, -FCS ratio: ###p < 0.0001; ##p < 0.002.

Back to article page