Twist 1 transcription was downregulated in the adipose tissue of obese subjects. (A/B) An obesity model was established in C57/BL6J mice that were fed a high-fat diet for 14 weeks. Twist 1 transcription was downregulated significantly in subcutaneous adipose tissue compared with its level in the control groups (*P < 0.05 vs. control). (C/D) An obesity model was established in Wistar rats that were fed a high-fat diet, and a reduced Twist 1 mRNA level was observed in subcutaneous adipose tissue (*P < 0.05 vs. control). (E/F) The level of Twist 1 transcription in the subcutaneous adipose tissues of individuals with a BMI ≥ 30 was lower than in non-obese individuals (*P < 0.05 vs. 20 < BMI < 25 group).