Figure 4From: Twist 1 regulates the expression of PPARγ during hormone-induced 3T3-L1 preadipocyte differentiation: a possible role in obesity and associated diseases3T3-L1 preadipocytes were induced to differentiate in vitro, and the dynamic expression of Twist 1 was detected. (A) Morphological observations and oil red O staining of 3T3-L1 preadipocytes and adipocytes. (B) Quantitative analysis of intracellular oil red O staining using spectrophotometry. (C) PPARγ and ALBP protein expression levels at different stages of 3T3-L1 preadipocyte differentiation. (D/F) The dynamic transcription of Twist 1 was detected at different stages of 3T3-L1 preadipocyte differentiation (*P < 0.05 vs. control, day 0 cells). (E/G) The dynamic expression of Twist 1 was detected at different stages of 3T3-L1 preadipocyte differentiation (*P < 0.05 vs. control, day 0 cells).Back to article page