The role of Twist 1 in adipogenesis was explored using retroviral interference of Twist 1 expression. (A/B) Retroviral interference of Twist 1 expression was conducted using LV3. Western blot analysis showed that Twist 1 expression was reduced to 70-80% of the control. (C/D) Oil red O staining was used to analyze the differentiation of 3T3-L1/NC and 3T3-L1/Twist 1- cells. The results showed that interference of Twist 1 did not influence the differentiation, and there were no significant differences among the groups. (E) The expression levels of the PPARγ and ALBP proteins were increased in the Twist 1 shRNA-treated cells compared with the vector-treated control cells under hormone-induced differentiation. The infection time for both Twist 1/shRNA and LV3/NC was 48 h.