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Figure 6 | Lipids in Health and Disease

Figure 6

From: Twist 1 regulates the expression of PPARγ during hormone-induced 3T3-L1 preadipocyte differentiation: a possible role in obesity and associated diseases

Figure 6

Retroviral interference of Twist 1 expression enhanced the expression of PPARγ on day 4 of hormone-induced differentiation. (A) Real-time PCR analysis revealed that PPARγ mRNA was upregulated on day 4 of hormone-induced differentiation after retroviral interference of Twist 1 expression compared with its expression level in 3T3-L1/NC cells (*P < 0.05 vs. day 0). (B/C) PPARγ protein expression on day 4 of hormone-induced differentiation was significantly enhanced when Twist 1 expression was downregulated; there were no obvious differences at the other time points (*P < 0.05 vs. 3T3-L1/NC cells at day 4). The infection time for both Twist 1/shRNA and LV3/NC was 48 h.

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