Skip to main content
Figure 6 | Lipids in Health and Disease

Figure 6

From: The rhythmic expression of clock genes attenuated in human plaque-derived vascular smooth muscle cells

Figure 6

Circadian expression of Per1, Per3 and Cry2 at mRNA levels in primary cultured VSMCs. (A) Circadian expression of Per1 mRNA in normal human carotid VSMCs and human plaque-derived VSMCs. (B) Circadian expression of Per3 mRNA in normal human carotid VSMCs and human plaque-derived VSMCs. (C) Circadian expression of Cry2 mRNA in normal human carotid VSMCs and human plaque-derived VSMCs. The mRNA levels were determined by qRT-PCR at the indicated time points after the serum shock. Values of Per1, Per3,and Cry2 mRNA were normalized to GAPDH mRNA. The signal levels at ZT0 of normal human carotid VSMCs were defined as 1. Each value represents the mean ± SD (n1 = 3 of normal human carotid VSMCs; n2 = 5 of human plaque-derived VSMCs). The differences of expression levels were assessed by unpaired Student’s t test. *p < 0.05 in normal human carotid VSMCs versus human plaque-derived VSMCs. **p < 0.01 in normal human carotid VSMCs versus human plaque-derived VSMCs.

Back to article page

Lipids in Health and Disease

ISSN: 1476-511X