Gene expression analysis of PUFA treated and irradiated glioma cell line. U87 MG cells were irradiated with 10 Gy, treated with polyunsaturated fatty acids (PUFAs) and incubated for 48 hours. Gene expression of c-MYC, TP53 (p53), potential therapeutic targets (NOTCH1, GADD45A, EGR1) and early response genes (EGR1,TNF-α, FOSL1, c-FOS) were determined with RT-PCR. Abbreviations: AA - 25 μM arachidonic acid; DHA - 25 μM docosahexaenoic acid; GLA - 50 μM gamma linolenic acid. Abbreviations: */** - significant (p < 0.05/ p < 0.01) difference between Ct values of control cells and treated cells. #/## - significant (p < 0.05/ p < 0.01) difference between Ct values of cells exposed to 10 Gy and U87 MG cells subjected to PUFAs and 10 Gy at the same time.