Figure 4

Effect of forced expression of CSE on the secretion profile of adipokines in mature 3T3-LI adipocytes. Mature 3T3-LI adipocytes were transfected with lentiviral vector expressing mouse CSE or empty vector for 72h. Transfected and control cells were then treated high glucose (25mmol/L) or normal glucose (5.5mmol/L) for48h. The culture media were then collected for assays of TNFα, MCP-1 and adiponectin (A, B, C). Cell lysates were used for Western blotting analysis of CSE protein expression (D). Results are shown as mean ± SD of three independent experiments. ^*P < 0.05, ^**P < 0.01, vs. NG alone. ^#P < 0.05, ^##P < 0.01, vs. HG alone.