Table 2 Summary of the primers and PCR-RFLP conditions used for the analysis of the APOB polymorphisms

SP Ins/Del (rs11279109)

5’-CAGCTGGCGATGGACCCGCCGA-3’

95°C 5 mins

93/84

N/A

Xu et al. [41]

95°C 0.50 sec, 65°C 1 min,

5’-ACCGGCCCTGGCGCCCGCCAGCA-3’

65°C 0.50 sec (35 cycles)

72°C 7 mins, hold at 4°C

Codon 2488 Xba1 (rs693)

5’-GATGAAACCAATGACAAAATCC-3’

94°C 5 mins

450

240

Pan et al. [31]

5’-AACAGTGAACCCTTGCTCTACC-3’

94°C 0.30 sec, 58°C 30 sec,

70°C 0.30 sec(40 cycles)

210

70°C 10 mins, hold at 4°C

Codon 3611 Msp1 (rs1801701)

94°C 5 mins

273

169

Pan et al. [31]

5’-GAGGAACCTTAGGTGTCCTTC-3’

94°C 0.30 sec, 56°C 30 sec,

70°C 0.30 sec(40 cycles)

104

70°C 10 mins, hold at 4°C

Codon 4154 EcoR1 (rs1042031)

5’-TAGGCAAATTGATGATATCGA-3’

94°C 5 mins

330

181

Pan et al. [31]

5’-ACCTGGGACAGTACCGTCCCTA-3’

94°C 0.30 sec, 54°C 30

sec,70°C 0.30 sec(40 cycles)

149

70°C 10 mins, hold at 4°C

3’VNTR

5’-6- FAM- ATGGAAACGGAGAAATTATG-3’

95°C 5 mins

500-900

N/A

Alonso et al. [30]

94°C 1 min, 58°C 1 min,

5’-CCTTCTCACTTGGCAAATAC-3’

72°C 4 mins (29 cycles)

72°C 7 mins, hold at 4°C