Figure 1

Proliferation, migration and loss of adhesion induced by LDL in in breast cancer cell lines. A, Number of cells (MDA MB 231, HTB 126, HTB 20) after 24 h and 48 h of LDL (100 μg/mg) exposure is consistently higher than control (non-treated) condition. B and C, Cells (MDA MB 231) were wounded and then cultured for 24 h, with LDL (100 μg/mL), HDL (100 μg/mL) or control conditions. Cell migration into the wound was examined by phase-contrast microscopy and migration distance is indicated as the percentage of the wound closure at 24 h. Representative photos are shown (original magnification 100x C). D and E, Number of cells (MDA MB 231) adherent and no adherent cells, at 4 h after being removed and reseeded on its primary conditions (control and LDL100 μg/mL) shows that LDL treated cells lose matrix adhesion compared to control. Representative photos are shown (original magnification 200x E). P value and the number of the experiments are represented in the figure. Columns mean; bars ± SEM.