Identification of a loss-of-function mutation in Ampd2 in hypercholesterolemic mice. A) Sequencing chromatographs of Ampd2 in Ampd2+/+, Ampd2+/m and Ampd2m/m mice. B) Liver immunoblot analysis of Ampd2 protein in Ampd2+/+, Ampd2+/m and Ampd2m/m mice. C) Amino acid conservation of mouse Ampd2 residues 321–361 to Ampd2+, mouse Ampd1, mouse Ampd3, rat Ampd2, human Ampd2, dog Ampd2, Caenorhabditis elegans Ampd, Arabidopsis thaliana AMPD and Saccharomyces cerevisiae Ampd. Arrow indicates residue 341.