Evaluation of the effects of endogenous female sex hormones removal on total plasma cholesterol levels. As expected, ApoE mice present hypercholesterolemia, which was further increased after ovariectomy. C57 Sham n = 7, C57 OVX n = 6, ApoE Sham n = 7, ApoE OVX n = 9. Values are means ± SEMs. **p < 0.01 vs. C57; ##p < 0.01 vs. respective control. Two-way ANOVA followed by Tukey’s post hoc test.