Western blot analysis of ER-α expression in the renal cortex (A) and medulla (B). (A): The removal of endogenous female sex hormones resulted in reduced ER-α expression in renal cortex in both C57 and ApoE groups. (B) Neither hypercholesterolemia nor ovariectomy affected medullary ER-α expression. All groups have n = 4. Values are means ± SEMs. #p < 0.05 vs. respective control. Two-way ANOVA followed by Tukey’s post hoc test.