Figure 6

Flow cytometric analysis of apoptosis in kidney cells. Each dot plot (A) was constructed using propidium iodide (PI) and annexin V/FITC staining indicating: damaged cells (Q1), cells that are undergoing late apoptosis (Q2), viable cells (Q3) and cells in early apoptosis (Q4). Bar graph B shows the average percentage of PI positive (viable) cells, which were used to quantify apoptosis. Bar graph C shows the average percentage of cells in early and late apoptosis in non-diabetic mice (ND), diabetic mice administered vehicle (DV) and diabetic mice treated with quercetin (DQ). Values are mean ± SEM for 6 to 8 animals per group. *p < 0.05 vs. ND group and ^#p < 0.05 vs. DV group.