Unavailability of liver triacylglycerol increases serum cholesterol concentration induced by dietary cholesterol in exogenously hypercholesterolemic (ExHC) rats

Table 2 Primers for real-time RT-PCR

Gene	Forward primer	Reverse primer	Product (bp)
Actb	TCAGGTCATCACTATCGGCA	TCATGGATGCCACAGGATTC	93
Mtp	CGACGGTGACGATGATCAACT	TGACCCGCATTTTCGACATT	66
Apob	ACAACCCTCACGGTCTTTGG	GAGACACGATCTGGAACTTG	171
Apoe	AGGAGCAGACCCAGCAGATA	GGAGTTGGTAGCCACAGAGG	143
Fasn	ATGCACACAGTGCTCAAAGG	GTATCCTCCACAGGCAGGAA	227
Scdl	GAGATACACTCTGGTGCTC	AAGGCGTGATGGTAGTTGTGG	156
Fads1	TCAGCGGAAGAAATGGGTG	GGATATGGTTCATCTGCGTCA	167
Fads2	TTGTCCTTGGAGAGTGGCAG	GTACATAGGGATGAGCAGCG	119
Dgat1	GATGCTCTTTTTCACCCAGC	GAGACGCTCAATGATTCGTG	118
Dgat2	CTGGCTGGCATTTGACTGGA	CTGGATGGGAAAGTAGTCTCG	108
Gk	GAATCCCACTCAGCCATTTG	TCCTAGAGCAGTTGTCTCGG	128
Gpdl	CAAACACCCAACTTCCGCATC	CAGCCCCAACAGCCACTATA	91
Ppap2a	GCGATGGCTACATTGAGAAC	GGCTTGAAGATAAAGTGCGAC	134
Srebf1	ATGCCATGGGCAAGTACACA	ACGTGTCAAGAAGTGCAAGG	179
Cptla	AAGGTGCTGCTCTCCTACCA	GGCCTCACAGATTCCAGGTA	193
Cpt2	TGACAGCCAGTTCAGGAGAA	ATACTCAGACTTTGGGTCCG	217
Soat1	GATGGGGTTATGTTGCTATGC	GGGCTCCTGTTTGATATTCCG	113
Acat1	CAGGTCTACCCATTGCCACT	CCCACCGTATGGTGTTGCTC	185
Ldlr	CACTGTGGCAGTAGTGAGTG	GGCTACCGTGAATACAGGAG	151
Vldlr	CCGTTCTACTCAGTGTATCCC	CGTCACAGTCATCCTGTCCA	176
Lipc	ACTCTTCCTCATCACCCGAG	CGCTGTTTTCCCACTTGAAC	257
Lsr	GAGGGTCCTATACTATATGGAG	TGGAGGGAGGTTACTTCACTC	108
Cd36	GAAGCACTGAAGAATCTGAAGAG	TCCAACACCAAGTAAGACCATC	159
Smek1	GAGCGACGGTTCTCTTCTTC	CAGACCACACAATCAGAGTGTC	86
Smek2	CTGCATATCAGAAGCAGCAG	ACTGATGGGTCCTTACCTTG	142

Gene symbols: Acat1, acetyl-CoA acyltransferase; Apob, apolipoprotein B; Apoe, apolipoprotein E; Cpt1a, CPT on mitochondrial outer membrane; Cpt2, CPT on mitochondrial inner membrane; Dgat1/2, diacylglycerol O-acyltransferase 1/2; Fads1, Δ5 fatty acid desaturase; Fads2, Δ6 fatty acid desaturase; Fasn, fatty acid synthetase; Gk, glycerol kinase; Gpd1, glycerol-3-phosphate dehydrogenase 1 (soluble); Mtp, microsomal triglyceride transfer protein; Ppap2a, phosphatidic acid phosphatase type 2A; Scd1, stearoyl-coenzyme A (CoA) desaturase; Srebf1, sterol regulatory element binding transcription protein 1; Soat1, acyl-CoA cholesterol acyltransferase. Uptake: Cd36, fatty acid translocase; Ldlr, low-density lipoprotein receptor; Lipc, hepatic lipase; Lsr, lipolysis-stimulated lipoprotein receptor; Smek1/2, homolog 1/2, suppressor of mek1 (Dictyostelium); Vldlr, very-low-density lipoprotein receptor.

ISSN: 1476-511X