CCB-diet impaired vascular reactivity of thoracic aortae by amending nitric oxide pathway. A) Isolated aorta experiment; PE: phenylephrine; Ach: acetyl choline, Two-way ANOVA followed by Bonferroni’s post-test is applied. B) Nitric oxide pathway; NO: nitric oxide (uM); eNOS: endothelial nitric oxide synthase; ND: normal diet; ND-F normal diet with two-hour fasting; CCB: cholesterol-cholate-butterfat diet; CCB-F: cholesterol-cholate-butterfat diet with two-hour fasting. All values are represented as mean ± standard error of mean (SEM) (n = 7 per group). This figure only shows the comparison of means using one-way ANOVA followed by Tuckey’s post-test (*p < 0.05, **p < 0.01 and ***p < 0.001).