Figure 3

miR-21 inhibited lipid accumulation by TLR4-NF-κB pathway. (A) After overexpressed or silenced the expression levels of miR-21, the activation of TLR4 and intra-nuclear NF-κB p65 was demonstrated by western blotting analysis. (B-C) Before stimulation with 100 ng/ml LPS for 24 h, cells were stimulated with anti-TLR4 antibody (10 μg/ml), or 30 μM NF-κB inhibitor PDTC for 4 h. The silencing effect of TLR4 (B) and intra-nuclear NF-κB p65 (C) was evaluated. (D) The association between miR-21 and the TLR4-NF-κB pathway was analyzed by western blotting. *P < 0.05.