Figure 3
Adipose tissue results. At the top Histological cuts of adipose tissue, Hematoxilin-eosin stain (A) EPA:DHA 1:1 in SHROB rat (100x), we can observe the variable size of adipocytes and intuit the presence of inflammatory cells. (B) EPA:DHA 1:2 in SHROB rat (300x) mast cells marked up by black arrows. At the bottom four graphics in which: (W) refers to Wistar strain, (S) refers to SHROB strain. (A) EPA:DHA 1:1, (B) EPA:DHA 2:1, (C) EPA:DHA 1:2. Equal letters, a, b, c, d, e means statistical differences among strains. (C) In both strain of rats there weren’t statistically significant differences in the proportion of different adipocyte sizes among the treatment groups. Comparing the two strains, it was higher in SHROB rats than in WISTAR rats for the EPA:DHA 1:1 diet (p = 0.031a). (D) As far as the presence of white cells, and therefore inflammation, is concerned; there was a higher effect of PUFA n-3 in Wistar rats than in SHROB rats. In these strain only EPA:DHA 1:2 treatment doesn’t show a significant improvement (p = 0.001c) (E) Mast cells % in Wistar rats showed differences with EPA:DHA 1:1 (p < 0.001*). And in SHROB rats (p = 0.022^, p = 0.018^ respectively) Comparing the two strains, in the EPA:DHA 1:1 diet, SHROB showed a greater presence of mast cells than WISTAR (p = 0.001b). (F) The proportion of macrophages in SHROB rats was lower than in Wistar rats but no significant differences were founded.