Figure 1From: The effects of obesity, diabetes and metabolic syndrome on the hydrolytic enzymes of the endocannabinoid system in animal and human adipocytesEndocannabinoid degradation enzyme assay validation. FAAH activity was detected in the total particulate and not cytosolic fraction of adipocyte homogenates of Zucker rats (A, n = 48), and was significantly inhibited by URB597 (1 μM, C, n = 48). MGL activity was detected in the cytosolic and not total particulate fraction of adipocyte homogenates (B, n = 33), and was significantly inhibited by MAFP (1 μM, D, n = 66). Data are given as means with error bars representing S.E.M and were analysed by a Mann–Whitney test (*** P < 0.001, *** P < 0.001).Back to article page