Figure 3

PC profile changes during copper-induced oxidation of human LDL treated with a PAF-AH inhibitor. Human LDL was pretreated with pefabloc, a PAF-AH inhibitor, and the treated LDL (0.2 mg/mL) was incubated with copper sulfate (5 μM) at 37°C for up to 3 h. Lipid extraction and LC-MS/MS analysis were performed as in Figure 2. The peak area (cps) for each species was normalized based on the peak areas of internal standard, and means and SD were calculated from six independent preparations. The 42 PC species were classified into five groups: PUFA-PC (A), S/MUFA-PC (B), lysoPC (C), cleaved-chain oxPC (D) and long-chain oxPC (E). Statistical significance of comparison with the sample without incubation (0 min) was calculated by ANOVA; *, p < 0.05; #, p < 0.01; +, p < 0.005; **, p < 0.001.