Effects of PAF-AH inhibitor pretreatment on changes in PC species during oxidation of human LDL. Human LDL and pefabloc-treated human LDL (0.2 mg/mL) were incubated with copper sulfate (5 μM) at 37°C for 3 h. Lipid extraction and LC-MS/MS analysis were performed, and the change in peak area (cps) during the 3 h oxidation was calculated for each PC species. The 42 PC species were classified into five groups: PUFA-PC (A), S/MUFA-PC (B), lysoPC (C), cleaved-chain oxPC (D) and long-chain oxPC (E). Values indicated are means and SD (n = 6). Statistical significance between the samples with and without treatment with PAF-AH inhibitor was calculated by Welch t-test; *, p < 0.05; #, p < 0.01; +, p < 0.005; **, p < 0.001.