Effect of the prolonged high-fat diet on the fatty acid metabolism in rat blood and liver

Zhukova, Natalia V; Novgorodtseva, Tatyana P; Denisenko, Yulia K

doi:10.1186/1476-511X-13-49

Lipids in Health and Disease

Table 2 The content of fatty acids (wt%) in plasma and erythrocytes of rats at a high-fat load

From: Effect of the prolonged high-fat diet on the fatty acid metabolism in rat blood and liver

Fatty acid	Source	High-fat load
Fatty acid	Source	30 days		90 days		180 days
		Control	Experiment	Control	Experiment	Control	Experiment
14:0	Erythrocyte	0.52 ± 0.04	0.83 ± 0.08 / 1.6↑*	0.48 ± 0.04	0.5 ± 0.06	0.50 ± 0.03	***1.01 ± 0.12 / 2↑
	Plasma PL	0.50 ± 0.04	*1.22 ± 0.20 / 2.2↑**	0.62 ± 0.04	0.57 ± 0.05	0.60 ± 0.07	0.37 ± 0.04 / 1.6↓**
	Plasma TAG	1.43 ± 0.10	1.71 ± 0.10 / 1.2↑*	1.50 ± 0.10	1.53 ± 0.15	1.48 ± 0.10	0.87 ± 0.11 / 1.7↓*
	Plasma ES	1.05 ± 0.06	1.23 ± 0.01	0.95 ± 0.09	0.84 ± 0.06	1.10 ± 0.05	*0.42 ± 0.05 / 2.5↓**
15:0	Erythrocyte	0.52 ± 0.04	0.58 ± 0.07	0.72 ± 0.06	0.43 ± 0.02 / 1.7↓**	0.76 ± 0.07	0.56 ± 0.04 / 1.4↓*
	Plasma PL	0.75 ± 0.06	*1.33 ± 0.07 / 1.7↑**	0.72 ± 0.05	*0.45 ± 0.02 / 1.6↓**	0.68 ± 0.05	*0.40 ± 0.01 / 1.7↓**
	Plasma TAG	0.92 ± 0.08	*1.55 ± 0.07 / 1.6↑**	0.90 ± 0.10	0.86 ± 0.05	0.94 ± 0.07	0.52 ± 0.08 / 1.8↓**
	Plasma ES	0.80 ± 0.02	*1.36 ± 0.09 / 1.7↑**	0.84 ± 0.04	***0.41 ± 0.03 / 2↓	0.93 ± 0.02	*0.35 ± 0.01 / 2.6↓**
16:0	Erythrocyte	23.72 ± 0.84	24.38 ± 0.57	24.12 ± 0.68	*28.6 ± 0.5 / 1.2↑**	24.72 ± 0.74	27.6 ± 1.4 / 1.1↑**
	Plasma PL	28.50 ± 0.33	27.68 ± 0.56	26.50 ± 0.43	25.64 ± 0.83	28.70 ± 0.30	27.02 ± 0.36
	Plasma TAG	28.16 ± 2.24	26.42 ± 0.68	27.60 ± 2.00	24.23 ± 1.66	28.86 ± 1.80	22.2 ± 0.52 / 1.3↓**
	Plasma ES	17.32 ± 3.49	15.67 ± 1.66	17.18 ± 1.00	*11.47 ± 0.43 / 1.5↓**	17.00 ± 0.09	*11.32 ± 0.12 / 1.5↓**
16:1n-7	Erythrocyte	1.90 ± 0.25	1.83 ± 0.12	1.88 ± 0.22	*0.83 ± 0.05 / 2.3↓**	2.04 ± 0.30	2.04 ± 0.21
	Plasma PL	1.2 ± 0.1	*3.31 ± 0.40 / 2.7↑**	1.1 ± 0.2	0.91 ± 0.07	1.1 ± 0.6	1.17 ± 0.14
	Plasma TAG	6.57 ± 0.34	8.67 ± 0.14 / 1.3↑*	7.56 ± 0.30	***2.53 ± 0.27 / 3↓	8.10 ± 0.46	*2.72 ± 0.39 / 3↓
	Plasma ES	5.35 ± 0.59	*8.14 ± 0.77 / 1.6↑**	5.20 ± 0.40	3.28 ± 57.14 / 1.6↓*	5.15 ± 0.90	4.75 ± 0.19
18:0	Erythrocyte	10.10 ± 0.50	14.68 ± 0.48 / 1.4↑**	10.7 ± 0.45	*17.3 ± 0.57 / 1.7↑**	9.9 ± 0.40	*13.9 ± 1.77 / 1.4↑**
	Plasma PL	19.00 ± 0.37	21.67 ± 0.94 / 1.1↑*	19.40 ± 0.67	*23.23 ± 0.14 / 1.2↑**	18.70 ± 0.70	18.97 ± 0.72
	Plasma TAG	5.00 ± 0.03	3.00 ± 0.16 / 1.6↓*	4.80 ± 0.10	*8.72 ± 0.91 / 1.8↑**	5.10 ± 0.30	4.92 ± 0.34
	Plasma ES	3.75 ± 0.54	3.72 ± 0.46	3.45 ± 0.94	3.35 ± 0.22	3.70 ± 0.50	3.15 ± 0.56
18:1n-9	Erythrocyte	7.66 ± 0.57	11.45 ± 1.22 / 1.5↑**	7.51 ± 0.70	10.5 ± 0.31 / 1.4↑**	8.16 ± 0.67	11.2 ± 1.38 / 1.4↑**
	Plasma PL	6.77 ± 0.41	7.67 ± 0.28	6.14 ± 0.50	6.91 ± 2.09	6.35 ± 0.60	9.52 ± 0.34 / 1.5↑*
	Plasma TAG	25.27 ± 2.26	26.5 ± 2.36	27.70 ± 2.20	30.66 ± 1.87	26.10 ± 1.20	*36.55 ± 1.14 / 1.4↑**
	Plasma ES	22.30 ± 3.7	***11.5 ± 0.9 / 2↓	21.64 ± 2.0	*36.8 ± 2.7 / 1.7↑**	21.30 ± 1.70	*46.5 ± 0.8 / 2.2↑**
18:2n-6	Erythrocyte	14.02 ± 0.64	*9.78 ± 0.23 / 1.4↓**	13.12 ± 0.64	12.6 ± 0.4	14.62 ± 0.84	11.3 ± 0.97 / 1.3↓**
	Plasma PL	18.50 ± 0.6	18.85 ± 1.05	18.9 ± 0.60	18.12 ± 1.21	17.8 ± 0.50	18.30 ± 0.20
	Plasma TAG	19.62 ± 2.86	*15.3 ± 1.1 / 1.2↓**	20.20 ± 2.00	20.23 ± 1.40	18.82 ± 1.80	17.12 ± 0.16 / 1.1↓*
	Plasma ES	19.25 ± 1.19	19.17 ± 1.35	20.25 ± 1.10	21.08 ± 0.96	18.40 ± 1.00	*13.12 ± 0.2 / 1.4↓**
20:3n-9	Erythrocyte	0.35 ± 0.12	tr	0.27 ± 0.10	0.17 ± 0.03	0.31 ± 0.10	***0.95 ± 0.09 / 3↑
	Plasma PL	0.55 ± 0.02	***0.27 ± 0.02 / 2↓	0.54 ± 0.10	***0.25 ± 0.09 / 2↓	0.62 ± 0.12	0.65 ± 0.02
	Plasma TAG	0.72 ± 0.05	*0.30 ± 0.01 / 2.4↓**	0.77 ± 0.08	0.73 ± 0.12	0.74 ± 0.06	***1.45 ± 0.34/ 2↑
	Plasma ES	0.96 ± 0.17	0.83 ± 0.31	0.94 ± 0.14	*0.45 ± 0.05 / 2.1↓**	0.89 ± 0.20	0.75 ± 0.17
20:3n-6	Erythrocyte	0.60 ± 0.13	0.88 ± 0.09	0.62 ± 0.10	**1.23 ± 0.06 / 2↑	0.70 ± 0.10	0.9 ± 0.08
	Plasma PL	1.77 ± 0.39	1.38 ± 0.12	1.82 ± 0.30	2.05 ± 0.14	2.10 ± 0.70	*3.05 ± 0.29
	Plasma TAG	0.77 ± 0.38	*0.27 ± 0.20 / 2.8↓**	0.67 ± 0.30	0.51 ± 0.06	0.70 ± 0.30	0.72 ± 0.19
	Plasma ES	0.42 ± 0.06	0.66 ± 0.08 / 1.5↑*	0.52 ± 0.10	0.55 ± 0.06	0.48 ± 0.09	0.47 ± 0.02
20:4n-6	Erythrocyte	6.78 ± 1.70	*21.27 ± 1.92 / 3.1↑**	6.80 ± 1.20	*16.3 ± 0.5 / 2.4↑**	6.90 ± 1.72	*15.9 ± 0.94 / 2.3↑**
	Plasma PL	12.2 ± 0.72	*7.56 ± 0.62 / 1.7↓**	12.8 ± 0.80	13.66 ± 0.72	13.0 ± 0.80	9.80 ± 0.60 / 1.3↓**
	Plasma TAG	2.45 ± 0.65	*0.87 ± 0.16 / 2.7↓**	2.50 ± 0.60	2.38 ± 0.25	2.20 ± 0.50	2.11 ± 0.32
	Plasma ES	28.73 ± 7.34	18.84 ± 2.73 / 1.5↓**	30.54 ± 3.30	*10.96 ± 0.86 / 2.8↓**	28.50 ± 2.40	*10.55 ± 0.90 / 2.7↓**
20:5n-3	Erythrocyte	0.90 ± 0.13	0.73 ± 0.09	0.86 ± 0.10	*0.58 ± 0.05	0.93 ± 0.12	**0.68 ± 0.05
	Plasma PL	0.42 ± 0.13	*1.55 ± 0.18 / 3.7↑**	0.48 ± 0.10	0.62 ± 0.11	0.50 ± 0.10	0.50 ± 0.10
	Plasma TAG	0.70 ± 0.03	*1.17 ± 0.11 / 1.6↑**	0.72 ± 0.08	0.92 ± 0.13 / 1.3↑*	0.73 ± 0.07	0.95 ± 0.05 / 1.3↑**
	Plasma ES	0.70 ± 0.20	tr	0.75 ± 0.10	*1.24 ± 0.12 / 1.7↑**	0.80 ± 0.20	0.95 ± 0.11
22:4n-6	Erythrocyte	0.58 ± 0.07	**1.58 ± 0.09 / 3↑	0.53 ± 0.07	0.85 ± 0.05 / 1.6↑**	0.56 ± 0.06	0.66 ± 0.08
	Plasma PL	0.15 ± 0.05	*0.33 ± 0.08 / 2↑	0.14 ± 0.07	*0.48 ± 0.06 / 3.2↑**	0.15 ± 0.05	0.15 ± 0.04
	Plasma TAG	0.30 ± 0.01	0.27 ± 0.01	0.20 ± 0.05	tr	0.31 ± 0.06	***0.61 ± 0.02 / 2↑
	Plasma ES	0.24 ± 0.04	tr	tr	tr	tr	tr
22:5n-6	Erythrocyte	0.23 ± 0.15	0.55 ± 0.05 / 2.5↑*	0.22 ± 0.10	*0.17 ± 0.03 / 1.3↓**	0.21 ± 0.12	*0.53 ± 0.13 / 2.5↑**
	Plasma PL	0.20 ± 0.01	tr	0.23 ± 0.05	*0.55 ± 0.01 / 2.4↑**	0.15 ± 0.05	tr
	Plasma TAG	tr	tr	tr	0.20 ± 0.01	tr	tr
	Plasma ES	0.10 ± 0.07	tr	tr	tr	tr	0.20 ± 0.01
22:5n-3	Erythrocyte	1.32 ± 0.16	1.62 ± 0.27	1.22 ± 0.14	1.38 ± 0.09	1.12 ± 0.17	1.15 ± 0.27
	Plasma PL	0.62 ± 0.13	*0.92 ± 0.10 / 1.5↑**	0.60 ± 0.15	0.61 ± 0.08	0.67 ± 0.20	0.65 ± 0.06
	Plasma TAG	0.50 ± 0.02	***1.05 ± 0.13 / 2↑	0.64 ± 0.08	0.74 ± 0.11	0.54 ± 0.06	***0.25 ± 0.07 / 2↓
	Plasma ES	0.15 ± 0.05	tr	tr	0.36 ± 0.09	tr	0.17 ± 0.02
22:6n-3	Erythrocyte	5.53 ± 0.12	3.93 ± 0.11 / 1.4↓*	4.53 ± 0.16	**2.22 ± 0.08 / 2↓	5.38 ± 0.17	*2.85 ± 0.53 / 1.9↓**
	Plasma PL	3.22 ± 0.47	4.45 ± 0.50	3.00 ± 0.70	3.27 ± 0.24	3.80 ± 0.80	4.27 ± 0.35
	Plasma TAG	1.45 ± 0.13	*2.93 ± 0.09 / 2↑	1.85 ± 0.40	2.46 ± 0.33	1.10 ± 0.10	***3.25 ± 0.32 / 3↑
	Plasma ES	1.65 ± 0.38	1.95 ± 0.11	1.35 ± 0.30	1.14 ± 0.12	1.74 ± 0.28	***0.87 ± 0.14 / 2↓

Mean ± m, n = 10.
Note: TAG, triacylglycerols; PL, phospholipids; ES, esters of sterols; tr, traces less than 0.1%. Asterisks indicate significant differences of fatty acid contents between rats from experimental group and corresponding control group: *for P < 0.05; **for P < 0.01, ***for P < 0.001. The numbers in boldface after the slash indicate the change of the value for the experimental group relative to that of the control group in the number of times. The arrow ↓ indicates that the value decreased. The arrow ↑ indicates that the value increased.

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ISSN: 1476-511X

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