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Figure 2 | Lipids in Health and Disease

Figure 2

From: Dihydrocapsaicin down-regulates apoM expression through inhibiting Foxa2 expression and enhancing LXRα expression in HepG2 cells

Figure 2

Effect of DHC on Foxa2 and expression in HepG2 cells. (A and B) HepG2 cells were divided into four groups and cultured in medium at 37°C containing 0 μM, 25 μM, 50 μM and 100 μM DHC for 24 h, respectively. Foxa2 transcript levels and protein levels were measured by real-time quantitative PCR and western blot analysis, respectively. (C) HepG2 cells were transfected with negative control or Foxa2 siRNA. And then protein samples were measured by western blot analysis. (D) HepG2 cells were transfected with Foxa2 or control siRNA and then incubated with 100 μM DHC for 24 h. And then protein samples were measured by western blot analysis. (E) HepG2 cells were transfected with pcDNA3.1-mock (control group) or pcDNA-Foxa2 (pcDNA group). And then protein samples were measured by western blot analysis. (F) HepG2 cells were transfected with pcDNA-Foxa2 or pcDNA-control and then incubated with 100 μM DHC for 24 h. Protein samples were measured by western blot analysis. All results are presented as mean ± SD of three independent experiments, each performed in triplicate. *P < 0.05 vs. the control group.

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Lipids in Health and Disease

ISSN: 1476-511X