Figure 3

Effect of DHC on LXRα and expression in HepG2 cells. (A and B) HepG2 cells were divided into four groups and cultured in medium at 37uC containing 0 μM, 25 μM, 50 μM and 100 μM DHC for 24 h, respectively. LXRα transcript levels and protein levels were measured by real-time quantitative PCR and western blot analysis, respectively. (C) HepG2 cells were transfected with negative control or LXRα siRNA. And then protein samples were measured by western blot analysis. (D) HepG2 cells were transfected with LXRα or control siRNA and then incubated with 100 μM DHC for 24 h. And then protein samples were measured by western blot analysis. (E) HepG2 cells were transfected with pcDNA3.1-mock (control group) or pcDNA-LXRα(pcDNA group). And then protein samples were measured by western blot analysis. (F) HepG2 cells were transfected with pcDNA-LXRα or pcDNA-control and then incubated with 100 μM DHC for 24 h. Protein samples were were measured by western blot analysis. All results are presented as mean ± SD of three independent experiments, each performed in triplicate. *P < 0.05 vs. the control group.