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Figure 3 | Lipids in Health and Disease

Figure 3

From: Linoleate appears to protect against palmitate-induced inflammation in Huh7 cells

Figure 3

Changes of IL8 mRNA expression following co-incubation with palmitate and linoleate. A. Dose-related changes of IL8 expression following co-incubation with 500 μM palmitate and linoleate (100 to 1000 μM) in Huh7 cells. Dose-related effect of linoleate in the regulation of palmitate-induced IL8 production was assessed following 9 hours of incubation. A 400 μM linoleate showed maximum suppression (n = 4, *p < 0.05). B. Suppressive effect of linoleate against IL8 production induced by palmitate in Huh7 cells. IL8 mRNA expression was significantly lower following co-incubation with palmitate and linoleate (7.33 fold increase; greater than the control, Huh7 cells incubated with bovine serum albumin) than those with palmitate treatment alone (133.5 fold increase, n = 6, *p < 0.0001). A co-incubation with 400μM linoleate showed suppression of IL8 expression with 94.5% decrease compared to the cells treated by 500μM palmitate alone. C. Dose-related changes of IL8 expression following co-incubation with 500 μM palmitate and linoleate (100 to 1000 μM) in HepG2 cells. Dose-related effect of linoleate in the regulation of palmitate-induced IL8 production was also assessed following 9 hours of incubation in HepG2 cells. A 300 μM linoleate showed maximum suppression with 87.1% decrease compared to the IL8 expression in the cells treated by 500 μM palmiate alone (n = 4, *p < 0.01).

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