Table 1 Methods and primers used in genotyping of TAFI −438 A/G, +1583 A/T, −1690 A/G and −2345 2G/1G polymorphisms
Gene polymorphisms | Analysis | Primers |
|---|---|---|
TAFI −438 A/G | PCR-RFLP | Forward:ACCAATGGGAATGTAGG |
Reverse:CCTTGCCTCTGACTTTT | ||
TAFI +1583 A/T | PCR-RFLP | Forward:CTTGGCATGTCATTAGG |
Reverse:TGCGGCATTTGTTGACA | ||
TAFI −1690 A/G | AS-PCR | Common Forward a:CACCTGTAGACTTTTGC |
Special Forward a1:TTAACTATTTTGACTGTTTA | ||
Special Forward a2:TTAACTATTTTGACTGTTTG | ||
Common Reverse b: CACTGAAGGAGAGAAAG | ||
TAFI −2345 2G/1G | AS-PCR | Common Forward a:CACCTCAACTGGACTATGT |
Special Forward a1:AGTTTTTAAAACATGAAAGA | ||
Special Forward a2:AGTTTTTAAAACATGAAAGGA | ||
Common Reverse b: TGTTCCCTTGCAGTTTAGC |