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Table 1 Primers and PCR conditions of four SNPs

From: CYP4F2 genetic polymorphisms are associated with coronary heart disease in a Chinese population

SNPs Primers Endonuclease Tm (°C)
rs2108622 F: 5’-ATCAACCCGTTCCCACCT-3’ Pvu II 55
R: 5’-ACATTGTGCTCCCAGACG-3’
rs3093100 F: 5’-AGTGCTTACTAGGGAACTGGAG-3’ Apa I 54
R: 5’-AAGGATTCAATGCAGGCCTGGA-3’
rs3093105 F: 5’-AGCCCTCCCTGCTCTACCT-3’ Eae I 56
R:5’-CCCACTCCCTAAGCCTCGT-3’;
rs3093135 F : 5’-GGCAGGCAGTCATCCACA-3’ Hinf I 52
R: 5’-CCAAACAGGCCCTCACAT-3’