Table 1 Primers and PCR conditions of four SNPs
From: CYP4F2 genetic polymorphisms are associated with coronary heart disease in a Chinese population
SNPs | Primers | Endonuclease | Tm (°C) |
|---|---|---|---|
rs2108622 | F: 5’-ATCAACCCGTTCCCACCT-3’ | Pvu II | 55 |
R: 5’-ACATTGTGCTCCCAGACG-3’ | |||
rs3093100 | F: 5’-AGTGCTTACTAGGGAACTGGAG-3’ | Apa I | 54 |
R: 5’-AAGGATTCAATGCAGGCCTGGA-3’ | |||
rs3093105 | F: 5’-AGCCCTCCCTGCTCTACCT-3’ | Eae I | 56 |
R:5’-CCCACTCCCTAAGCCTCGT-3’; | |||
rs3093135 | F : 5’-GGCAGGCAGTCATCCACA-3’ | Hinf I | 52 |
R: 5’-CCAAACAGGCCCTCACAT-3’ |