Figure 4

Effects of hexane fraction on the expression of lipid metabolism-related genes in 3T3-L1 cells. The cells were cultured in the presence of an ethanol vehicle only (); the hexane fraction at 500 μg/mL (); the hexane fraction at 1,000 μg/mL () during the differentiation induction period (Day 0-Day 2). All cells were harvested at Day 2 to determine the mRNA levels of the interested genes. Their relative expression levels were estimated in terms of their fold-change compared to the β-actin mRNA. Values are shown as a mean value ± S.D. of three-replicateexperimentsfor each treatment. Asterisks indicate a significant difference at p <0.05 from the untreated control (0 μg/mL). (ND = non-differentiated 3T3-L1 cells).