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Table 1 Primer sequences for Real-Time PCR

From: Ivy gourd (Coccinia grandis L. Voigt) root suppresses adipocyte differentiation in 3T3-L1 cells

Gene Forward sequence Accession No.
(product) Reverse sequence  
Actin 5′-CAGAAGGAGATTACTGCTCTGGCT-3′ NM_007393
(93 bp) 5′-GGAGCCACCGATCCACACA-3′  
ACC 5′-GGACCACTGCATGGAATGTTAA-3′ AY451393
(73 bp) 5′-TGAGTGACTGCCGAAACATCTC-3′  
Adiponectin 5′-GTTCCCAATGTACCCATTCGC-3′ NM_009605
(88 bp) 5′-TGTTGCAGTAGAACTTGCCAG-3′  
Adipor1 5′-TCTTCGGGATGTTCTTCCTGG-3′ NM_028320
(104 bp) 5′-TTTGGAAAAAGTCCGAGAGACC-3′  
C/EBPα 5′-TGGACAAGAACAGCAACGAGTAC-3′ AM_007678
(257 bp) 5′-GCAGTTGCCCATGGCCTTGAC-3′  
FABP4 5′-AGCATCATAACCCTAGATGG-3′ NM_024406.2
(115 bp) 5′-CATAACACATTCCACCACCAGC-3′  
FAS 5′- TGCTCCCAGCTGCAGGC -3′ AF_127033
(91 bp) 5′-GCCCGGTAGCTCTGGGTGTA-3′  
GLUT4 5′-CTGCAAAGCGTAGGTACCAA-3′ BC014282
(87 bp) 5′-CCTCCCGCCCTTAGTTG-3′  
IRS1 5′-CCAGAGTCAAGCCTCACACA-3′ NM_010570.4
(179 bp) 5′-GAAGACTGCTGCTGCTGTTG-3′  
LPL 5′-AGGGCTCTGCCTGAGTTGTA-3′ NM_008509
(199 bp) 5′-AGAAATCTCGAAGGCCTGGT-3′  
MEST 5′-GTTTTTCACCTACAAAGGCCTACG-3′ NM_008590
(52 bp) 5′-CACACCGACAGAATCTTGGTAGAA-3′  
PDK4 5′-GAGAAGAGCCCAGAAGACCA-3′ NM_013743
(134 bp) 5′-TCCACTGTGCAGGTGTCTTT-3′  
PPARγ 5′-AGGCCGAGAAGGAGAAGCTGTTG-3′ NM_011146
(276 BP) 5′-TGGCCACCTCTTTGCTGTGCTC-3′  
PPARγ1 5′-AAGATTTGAAAGAAGCGGTGAAC-3′ NM_001127330
(116 bp) 5′-CAATGGCCATGAGGGAGTTAG-3′  
SIRT1 5′-GACGACGAGGGCGAGGAG-3′ NM_019812
(79 bp) 5′-ACAGGAGGTTGTCTCGGTAGC-3′  
  1. ACC, acetyl-CoA carboxylase; Actin, cytoplasmic β-actin; Adipor1, adiponectin receptor-1; C/EBPα, CCAAT/enhancer binding protein transcription factor-α; FABP4, fatty acid binding protein-4; FAS, fatty acid synthase; GLUT4, glucose transporter-4; IRS1, insulin receptor substrate-1; LPL, lipoprotein lipase; MEST, mesoderm specific transcript; PDK4, pyruvate dehydrogenase kinase-4; PPARγ, peroxisome proliferator activated receptor-γ; PPARγ1, peroxisome proliferator activated receptor-γ1; SIRT1, sirtuin (silent mating type information regulation 2 homolog)-1; bp, base pairs.