Table 1 Primer sequences for Real-Time PCR
From: Ivy gourd (Coccinia grandis L. Voigt) root suppresses adipocyte differentiation in 3T3-L1 cells
Gene | Forward sequence | Accession No. |
|---|---|---|
(product) | Reverse sequence | |
Actin | 5′-CAGAAGGAGATTACTGCTCTGGCT-3′ | NM_007393 |
(93 bp) | 5′-GGAGCCACCGATCCACACA-3′ | |
ACC | 5′-GGACCACTGCATGGAATGTTAA-3′ | AY451393 |
(73 bp) | 5′-TGAGTGACTGCCGAAACATCTC-3′ | |
Adiponectin | 5′-GTTCCCAATGTACCCATTCGC-3′ | NM_009605 |
(88 bp) | 5′-TGTTGCAGTAGAACTTGCCAG-3′ | |
Adipor1 | 5′-TCTTCGGGATGTTCTTCCTGG-3′ | NM_028320 |
(104 bp) | 5′-TTTGGAAAAAGTCCGAGAGACC-3′ | |
C/EBPα | 5′-TGGACAAGAACAGCAACGAGTAC-3′ | AM_007678 |
(257 bp) | 5′-GCAGTTGCCCATGGCCTTGAC-3′ | |
FABP4 | 5′-AGCATCATAACCCTAGATGG-3′ | NM_024406.2 |
(115 bp) | 5′-CATAACACATTCCACCACCAGC-3′ | |
FAS | 5′- TGCTCCCAGCTGCAGGC -3′ | AF_127033 |
(91 bp) | 5′-GCCCGGTAGCTCTGGGTGTA-3′ | |
GLUT4 | 5′-CTGCAAAGCGTAGGTACCAA-3′ | BC014282 |
(87 bp) | 5′-CCTCCCGCCCTTAGTTG-3′ | |
IRS1 | 5′-CCAGAGTCAAGCCTCACACA-3′ | NM_010570.4 |
(179 bp) | 5′-GAAGACTGCTGCTGCTGTTG-3′ | |
LPL | 5′-AGGGCTCTGCCTGAGTTGTA-3′ | NM_008509 |
(199 bp) | 5′-AGAAATCTCGAAGGCCTGGT-3′ | |
MEST | 5′-GTTTTTCACCTACAAAGGCCTACG-3′ | NM_008590 |
(52 bp) | 5′-CACACCGACAGAATCTTGGTAGAA-3′ | |
PDK4 | 5′-GAGAAGAGCCCAGAAGACCA-3′ | NM_013743 |
(134 bp) | 5′-TCCACTGTGCAGGTGTCTTT-3′ | |
PPARγ | 5′-AGGCCGAGAAGGAGAAGCTGTTG-3′ | NM_011146 |
(276 BP) | 5′-TGGCCACCTCTTTGCTGTGCTC-3′ | |
PPARγ1 | 5′-AAGATTTGAAAGAAGCGGTGAAC-3′ | NM_001127330 |
(116 bp) | 5′-CAATGGCCATGAGGGAGTTAG-3′ | |
SIRT1 | 5′-GACGACGAGGGCGAGGAG-3′ | NM_019812 |
(79 bp) | 5′-ACAGGAGGTTGTCTCGGTAGC-3′ |