Semi-quantitative 3-D immunomicroscopy for cerebrovascular and neuronal inflammation. Cerebrovascular and neuronal inflammation was assessed by 3-D immuno quantitative analysis of the cerebral parenchymal and vascular expressions of GFAP and COX-2 in cortex (CTX) and hippocampus (HPF) of mice fed with low-fat (LF) or saturated fat (SFA) diet with/without probucol for 3 and 12 months. (A) Mean voxel intensity of parenchymal GFAP and COX-2 in CTX and HPF is shown. * indicates the statistical significance assessed with one-way ANOVA followed by Tukey’s post hoc test (p < 0.05, n = 8). (B) Pearson’s correlation coefficient between the GFAP or COX-2 expression and cerebral IgG/apoB extravasation in each animal was also performed (n = 24).