Reference memory task in the Morris water maze (MWM). Reference memory performance in the MWM of male control and ID + n-3 FAD rats repleted with an Fe + DHA/EPA, ID + DHA/EPA, Fe + n-3 FAD, or an ID + n-3 FAD diet for 5 weeks. (A) Mean distance moved per day to reach hidden platform located at same positions across 4 days, with 4 trials per day in the reference memory task; (B) Mean overall distance moved in the reference memory task. Values are means ± SEM, n = 7–8. #P <0.05 versus Fe + ALA group. ‡P <0.05 versus Fe + n-3 FAD group. ¥P <0.05 versus ID + n-3 FAD group. ALA, alphal-linolenic acid; n-3 FAD, n-3 fatty acid deficient; Fe, iron; ID, iron deficient.