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Table 3 Monoamine concentration (pmol/mg tissue) in three selected brain regions of male control rats and ID + n-3 FAD rats repleted with an Fe + ALA, ID + ALA, Fe + n-3 FAD, or an ID + n-3 FAD diet for 5 weeks

From: Providing male rats deficient in iron and n-3 fatty acids with iron and alpha-linolenic acid alone affects brain serotonin and cognition differently from combined provision

  p Value
pmol/mg tissue Control Fe + ALA ID + ALA Fe + n-3 FAD ID + n-3 FAD Fe ALA Fe × ALA
DA
FC 0.19 ± 0.06 0.40 ± 0.10 0.37 ± 0.09 0.38 ± 0.07 0.55 ± 0.10* 0.46 0.36 0.31
OB 1.28 ± 0.56 0.58 ± 0.07 0.63 ± 0.16 1.02 ± 0.21 0.73 ± 0.19 0.35 0.09 0.30
Str 6.87 ± 1.58 8.25 ± 2.56 9.10 ± 2.53 6.86 ± 2.73 7.68 ± 1.58 0.40 0.74 0.88
DOPAC
FC 2.22 ± 0.23 3.03 ± 0.50 3.25 ± 0.37 2.28 ± 0.33 3.27 ± 0.50 0.13 0.26 0.31
OB 2.99 ± 0.44 3.35 ± 0.35 2.99 ± 0.25 2.84 ± 0.20 3.51 ± 0.36 0.54 0.88 0.13
Str 12.78 ± 1.00 13.55 ± 0.87 11.97 ± 1.53 13.67 ± 2.37 11.86 ± 0.78 0.64 0.72 0.53
HVA
FC 1.04 ± 0.12 0.99 ± 0.21 1.29 ± 0.17 1.27 ± 0.21 1.26 ± 0.22 0.44 0.56 0.37
OB 2.22 ± 0.17 1.93 ± 0.19 1.90 ± 0.13 2.12 ± 0.10 2.28 ± 0.25 0.83 0.11 0.66
Str 16.26 ± 1.24 14.69 ± 1.14 14.19 ± 1.13 15.05 ± 1.32 14.42 ± 1.00 0.72 0.82 0.96
5-HT
FC 3.30 ± 0.66 1.97 ± 0.15 3.93 ± 0.74 4.67 ± 0.76# 3.36 ± 0.63 0.70 0.031 0.005
OB 2.37 ± 0.79 2.29 ± 0.50 2.76 ± 0.60 4.29 ± 0.55*,¥,# 2.22 ± 0.63 0.10 0.34 0.039
Str 2.58 ± 0.66 2.74 ± 0.46 2.51 ± 0.44 1.77 ± 0.32 2.34 ± 0.52 0.67 0.15 0.38
5-HIAA
FC 2.31 ± 0.28 2.40 ± 0.29 1.67 ± 0.23 2.28 ± 0.31 2.21 ± 0.31 0.15 0.46 0.23
OB 2.16 ± 0.37 2.15 ± 0.41 2.54 ± 0.21 1.75 ± 0.35 1.73 ± 0.27 0.42 0.16 0.26
Str 2.48 ± 0.51 7.00 ± 1.68 5.10 ± 0.88 4.09 ± 1.21 5.79 ± 1.15 0.68 0.30 0.20
NE
FC 1.58 ± 0.55 1.92 ± 0.42 1.45 ± 0.43 2.22 ± 0.56 1.40 ± 0.32 0.14 0.76 0.78
OB 2.77 ± 0.80 3.75 ± 0.85 2.27 ± 0.32 2.02 ± 0.29 2.35 ± 0.40 0.54 0.31 0.26
Str 2.70 ± 0.60 3.16 ± 0.55 1.91 ± 0.36 3.25 ± 0.68 2.18 ± 0.39 0.05 0.72 0.71
  1. Values are means ± SEM, n = 8–10 per group. Abbreviations: ALA, alpha-linolenic acid; DA, dopamine; n-3 FAD, n-3 fatty acid deficient; 5-HIAA, hydroxyindoleacetic acid; 5-HT, serotonin; DOPAC, dihydroxyphenylacetic acid; FC, frontal cortex; Fe, iron; HVA, homovanillic acid; ID, iron deficient; NE, norepinephrine; OB, olfactory bulb; Str, striatum.
  2. p Values correspond to two-way ANOVA to test effects of dietary Fe (deficient vs. sufficient) and dietary ALA (deficient vs. sufficient), and Fe × ALA interactions.
  3. *P <0.05 versus control group, analyzed using 1-way ANOVA with diet as between-subject variable followed by post hoc Dunnett’s test to determine whether experimental (repletion) groups differed from age-matched positive-control rats after repletion with Fe and/or ALA.
  4. #P <0.05 versus Fe + ALA group; P <0.05 versus Fe + n-3 FAD group; ¥P <0.05 versus ID + n-3 FAD group; Analyzed using 1-way ANOVA with diet (excluding control group) as between-subject variable followed by post hoc Tukey’s test to determine differences between experimental (repletion) groups.