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Table 4 Composition of experimental diets during depletion period *

From: Providing male rats deficient in iron and n-3 fatty acids with iron and alpha-linolenic acid alone affects brain serotonin and cognition differently from combined provision

Ingredient

g/kg diet

Casein, vitamin free

200

Carbohydrate

600

Cornstarch

368

Sucrose

100

Deytrose

132

Cellulose

50

Mineral mix#

35

Vitamin mix

10

L-Cystein

3

Choline bitartrate

2.5

TBHQ

0.02

Fat

100

 

n-3 FAD †

ALA sufficient ‡

Control ¥

Coconut oil (hydrogenated)

81.0

60.0

30.0

Soybean oil

0.0

0.0

70.0

Safflower oil

19.0

32.0

0.0

Flaxseed oil

0.0

8.0

0.0

  1. *Experimental diets were based on the AIN-93G formulation with modifications in iron content and fat source.
  2. #The mineral mix provided 35 mg iron/kg diet in the iron sufficient diets and 3 mg iron/kg diet in the iron deficient diets. n-3 FAD, n-3 fatty acid deficient; ID, iron deficient; TBHQ, t-butylhydroquinone.
  3. †Providing approximately 15.1 g/kg diet linoleic acid (LA) and 0.04 g/kg diet α-linolenic acid (ALA).
  4. ‡Providing approximately 20.0 g/kg diet LA and 46.0 g/kg diet ALA.
  5. ¥AIN-93G formulation providing approximately 35.7 g/kg diet LA and 4.8 g/kg diet ALA.