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Table 4 Composition of experimental diets during depletion period *

From: Providing male rats deficient in iron and n-3 fatty acids with iron and alpha-linolenic acid alone affects brain serotonin and cognition differently from combined provision

Ingredient g/kg diet
Casein, vitamin free 200
Carbohydrate 600
Cornstarch 368
Sucrose 100
Deytrose 132
Cellulose 50
Mineral mix# 35
Vitamin mix 10
L-Cystein 3
Choline bitartrate 2.5
TBHQ 0.02
Fat 100
  n-3 FAD ALA sufficient Control ¥
Coconut oil (hydrogenated) 81.0 60.0 30.0
Soybean oil 0.0 0.0 70.0
Safflower oil 19.0 32.0 0.0
Flaxseed oil 0.0 8.0 0.0
  1. *Experimental diets were based on the AIN-93G formulation with modifications in iron content and fat source.
  2. #The mineral mix provided 35 mg iron/kg diet in the iron sufficient diets and 3 mg iron/kg diet in the iron deficient diets. n-3 FAD, n-3 fatty acid deficient; ID, iron deficient; TBHQ, t-butylhydroquinone.
  3. Providing approximately 15.1 g/kg diet linoleic acid (LA) and 0.04 g/kg diet α-linolenic acid (ALA).
  4. Providing approximately 20.0 g/kg diet LA and 46.0 g/kg diet ALA.
  5. ¥AIN-93G formulation providing approximately 35.7 g/kg diet LA and 4.8 g/kg diet ALA.