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Figure 3 | Lipids in Health and Disease

Figure 3

From: Atorvastatin helps preserve pancreatic β cell function in obese C57BL/6 J mice and the effect is related to increased pancreas proliferation and amelioration of endoplasmic-reticulum stress

Figure 3

Atorvastatin upregulates PDX-1 and LXR- β expression and downregulates the protein expressions of ER stress markers. Total RNA was extracted from the pancreas of C57 mice and analyzed by quantitative real-time PCR. A comparative threshold cycle (CT) method was used for relative quantification of gene expression using beta-actin for normalization. Measurements were carried out in triplicate for each sample. (A) Relative mRNA levels of PDX-1 and LXR-β in pancreatic cells. Western blot analysis of pancreatic (B) PDX-1 (C) phosphorylated eIF2α (D) ATF4 (E) CHOP in C57 mice compared in three groups. Beta actin served as loading control. Data represented the mean of at least three independent experiments ± S.E.M. **p < 0.01, ***p < 0.001 vs. control.

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Lipids in Health and Disease

ISSN: 1476-511X