Effect of omega-3 polyunsaturated fatty acids on the cytoskeleton: an open-label intervention study

Table 2 Fluorescence intensity of phalloidin-labeled filamentous actin in red blood cells

	Fluorescence intensity of whole cell
Subject	t₀	t₁₂	p-values^a)	Difference (t₁₂– t₀)
1	60.17 ± 18.78	65.68 ± 7.93	0.401	5.52
2	84.25 ± 4.92	37.91 ± 7.97	< 0.001	-46.35
3	52.12 ± 19.86	61.71 ± 11.37	0.252	9.58
4	39.93 ± 8.47	30.15 ± 8.03	0.054	-9.79
5	25.61 ± 8.35	37.76 ± 9.99	0.014	12.16
6	52.02 ± 13.51	64.95 ± 4.91	0.028	12.93
7	31.89 ± 8.27	23.47 ± 7.61	0.056	-8.42
8	36.11 ± 14.67	47.56 ± 21.59	0.263	11.45
9	42.87 ± 18.46	58.42 ± 15.92	0.027	15.55
10	67.53 ± 6.09	46.77 ± 19.22	0.020	-20.77
11	66.88 ± 9.87	62.84 ± 11.22	0.387	-4.05
12	59.17 ± 5.32	21.24 ± 11.67	< 0.001	-37.94
	t ₀	t ₁₂
Group average ^b)	51.55 ± 20.28	46.54 ± 19.67	0.040	-5.01

Ten images were taken of each subject sample (n = 12) at each time point. Therefore, a total of 240 images (120 cells at each time point (t₀ and t₁₂)) were analyzed by Fiji software package [30]. Firstly, all images were calibrated for intensity ranges. Secondly, all images were filtered by a 2 μm median filter. An optimal threshold was selected by multiple testing and applied to all images (selected threshold: Li). After this post-processing, the overall fluorescence intensity of phalloidin-labeled filamentous actin in each cell was measured. Results are presented as mean ± SD. Statistically significant differences are marked in bold.
^a)t₀ values vs. t₁₂ values of subjects were tested by paired student’s t-test.
^b)n = 120.

ISSN: 1476-511X