Figure 5
From: Arachidonoyl-Phospholipid Remodeling in Proliferating Murine T Cells
AA content within PC and PE molecular species of proliferating and quiescent T cells. Splenic T cells, previously cultured as above for 48 hours with (open bars) or without (closed bars) anti-CD3 mAb, were harvested and PC and PE species were resolved by TLC. Phospholipids were further separated into diacyl-, 1-alkyl-2-acyl- and 1-alk-1'enyl-2-acyl-diaglycerides. Fatty acids were derivatized to methyl esters as above and arachidonoyl-methyl ester content was determined by gas chromatography. Data are the mean and standard error of 3 experiments.