Figure 7

Movement of [³H]AA within PC and PE species of proliferating and unstimulated T cells. Splenic T cells pooled from 6 mice, cultured as above for 48 hr with (open bars) or without (closed bars) anti-CD3 mAb, were labeled with [³H]AA (1 μCi/ml) for 20 min. The cells were then washed and cultured at 37°C for 60 min in BME with 1% BSA. At the initiation (zero time) and conclusion of culture, cells were harvested and PC and PE species were resolved by TLC. Phospholipids were further separated into diacyl-, 1-alkyl-2-acyl- and 1-alk-1'enyl-2-acyl-diaglycerides and the content of diacyl- and ether-lipids was quantified by liquid scintillation counting. Data are the mean and standard error of 3 experiments. *p < .05 compared to arachidonoyl phospholipid content at the initiation of culture. **p < .01 compared to arachidonoyl phospholipid content at the initiation of culture.