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Table 5 HMG-CoA reductase activity in hamster hepatic microsomes (pmol [14C]mevalonolactone/min/mg microsomal protein)

From: Cholesterol-lowering properties of Ganoderma lucidum in vitro, ex vivo, and in hamsters and minipigs

Group Activity-NaF Total activity+NaF
Control 12.36 9.30
Lovastatin 13.34 ND
Gl (2.5%) 5.86a 2.66a
Gl (5%) 8.08a 4.86a
  1. Refer to the text for methodologic details. [14C]mevalonate was converted to [14C]mevalonolactone with 10 M HCl before the TLC step. Values above were corrected for recovery (15–63%) with [3H]mevalonate. G. lucidum, but not lovastatin, was found to inhibit ex vivo synthesis whether: recovery was accounted for; results were expressed per gram liver weight; [14C]mevalonolactone was first extracted into organic solvent and then applied to TLC plates [79] rather than applying aqueous extracts directly to TLC plates [55] as reported above (data not shown); and whether or not endogenous phosphatase activity was inhibited with 50 mM NaF (shown above). Values represent mean of 6 determinations. aSignificantly different from control, students unpaired, 2-tailed t-test, equal variances (P < 0.05). ND, not determined.