Phosphoinositides synthesis in the liver of rats of different thyroid status. Panel A shows myo-[3H]inositol incorporation into the lipids of control rats. Panel B shows the lipid precursor incorporation into the lipids of liver of MMI-treated animals. Panel C shows effect of L-T4 on phosphoinositides synthesis in liver of MMI-treated rats. Treatment of the 90-day-old rats by MMI was performed as described in ''Materials and Methods''. L-T4 (200 μg/100 g weight) was injected to the MMI-treated rats 48 h prior to killing. Control rats received the same volume of 0.9% NaCl. Liver slices were incubated in the presence of the myo-[3H]inositol and lipids were extracted and separated as described in ''Materials and Methods'' and the radioactivity was determined by liquid scintillation counter. Results are mean ± S.E. of 6 – 8 individual experiments performed in duplicate. *P < 0.05 vs. control, **P < 0.05 vs. MMI-treated rats.