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Figure 3 | Lipids in Health and Disease

Figure 3

From: Effects of thyroxine and 1-methyl, 2-mercaptoimidazol on phosphoinositides synthesis in rat liver

Figure 3

Incorporation of [3H]arachidonic and [14C]oleic acid into liver total phospholipids and phosphoinositides of rats of different thyroid status. Panel A and B show [3H]arachidonic acid incorporation into total phospholipids and phosphoinositides of rats of different thyroid status, respectively. Panel C and D show the [14C]oleic acid incorporation into total phospholipids and phosphoinositides of rats different thyroid status, respectively. Treatment of the 90-day-old rats by MMI was performed as described in "Materials and Methods". L-T4 (200 μg/100 g weight) was injected to the MMI-treated rats 48 h prior to killing. Control rats received the same volume of 0.9% NaCl. Liver slices were incubated in the presence of the [3H]arachidonic or [14C]oleic acid and lipids were extracted and separated as described in ''Materials and Methods'' and the radioactivity was determined by liquid scintillation counter. Results are mean ± S.E. of 6 – 8 individual experiments performed in duplicate. *P < 0.05 vs. control, **P < 0.05 vs. MMI-treated rats.

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