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Figure 2 | Lipids in Health and Disease

Figure 2

From: NFY interacts with the promoter region of two genes involved in the rat peroxisomal fatty acid β-oxidation: the multifunctional protein type 1 and the 3-ketoacyl-CoA B thiolase

Figure 2

A. Competition between the 115 bp MFP-1 fragment and two different competitors. Unlabelled competitor NFY25 (lanes 2 to 5) or NFY mut (lanes 7 to 10) was added to the radiolabelled DNA probe and the rat liver nuclear extract. Lanes 1 and 6 contain only a 115 bp radiolabelled fragment with the nuclear extract. B. Competition between the oligonucleotides NFY-1 or NFY-2 of ThB and different competitors. Unlabelled competitor NFY-1 (lanes 2 to 4) or NFY-1 mut (lane 5) was added to the radiolabelled DNA probe and the rat liver nuclear extract. Unlabeled competitor NFY-2 (lanes 6 to 9) or NFY-2 mut (lane 10) was added to the radiolabelled DNA probe and the nuclear extract. Lanes 1 and 6 contain only radiolabelled oligonucleotides NFY-1 and NFY-2 respectively with the nuclear extract.

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