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Figure 2 | Lipids in Health and Disease

Figure 2

From: Inducible nitric oxide synthase links NF-κB to PGE2 in polyunsaturated fatty acid altered fibroblast in-vitro wound healing

Figure 2

NO production in 3T3-Swiss fibroblasts (n = 3, ± SD). Cells were incubated for 48 hr with bovine serum albumin alone as control or bovine serum albumin-soap loaded fatty acids (25 μM). Medium was then replaced with fresh fatty acid enriched medium containing lipopolysaccharide (LPS, 10 μg/ml) with or without NG-nitro-L-arginine methyl ester (L-NAME, 10-7 M) for another 24 hr. The wound was created after initial 48 hr treatments in a duplicate set of plate with LPS treatments. Culture supernatants (100 μl) were collected at 24 hr post wounding and quantification of nitrite was performed. The results were presented as the nitrite concentration (μM). Bars with different letters are significantly different (P < 0.05). EPA, eicosapentaenoic acid; AA, arachidonic acid; LPS, lipopolysaccharide; L-NAME, NG-nitro-L-arginine methyl ester.

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